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Quantitative Polymerase Chain Reaction (qPCR)-Based Rapid Diagnosis of Helicobacter pylori Infection and Antibiotic Resistance
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Genotypes of Helicobacter pylori in children with upper abdominal pain

Manisha Singh1, Kashi Nath Prasad, Surender Kumar Yachha

  • 1Department of Microbiology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow-226 014, India.

Journal of Gastroenterology and Hepatology
|August 13, 2003
PubMed

Insights

Helicobacter pylori infection is strongly linked to upper abdominal pain in children. Specific vacA genotypes, namely s1a/m1, are significantly associated with this condition in pediatric patients.

Area of Science:

  • Pediatric Gastroenterology
  • Infectious Diseases
  • Microbiology

Background:

  • Limited research exists on Helicobacter pylori (H. pylori) genotypes and their association with upper abdominal pain (UAP) in children.
  • This study prospectively investigated the link between H. pylori infection and UAP in a pediatric cohort.
  • The study also aimed to identify specific H. pylori vacA genotypes associated with UAP in children.

Purpose of the Study:

  • To determine the association between H. pylori infection and UAP in children.
  • To evaluate the role of specific vacA genotypes in pediatric UAP.
  • To compare H. pylori prevalence and genotypes in children with and without UAP.

Main Methods:

  • A prospective study involving 34 children with UAP and 110 controls without UAP.
  • H. pylori infection was assessed using antral biopsies via culture, rapid urease tests, histopathology, and ureA PCR.
  • Genotyping of H. pylori strains (cagA and vacA) was performed on 52 isolates.

Main Results:

  • A significant association was found between H. pylori infection and UAP (61.8% vs 28.2%, P = 0.0004).
  • The cagA gene was present in most H. pylori strains from both groups (95.2% with UAP, 90.3% controls).
  • Specific vacA alleles (s1a, m1) and the s1a/m1 genotype were significantly associated with UAP (P=0.018, P=0.015, P=0.007 respectively).

Conclusions:

  • H. pylori infection is a significant factor associated with UAP in children.
  • The vacA s1a/m1 genotype is strongly linked to UAP in pediatric patients.
  • The vacA s2, m2 alleles and s2/m2 genotype appear to play a minimal role in pediatric UAP.
Abstract

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