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Association between interleukin-18 gene polymorphism 105A/C and asthma.
1Department III of Internal Medicine, Osaka University Medical School, Osaka Department of Pediatrics, Fukui Medical University School of Medicine, Fukui, Japan.
Genetic variations in the Interleukin-18 (IL-18) gene were investigated. A specific polymorphism, 105A/C, was found more frequently in asthma patients, suggesting a potential link to asthma development.
Area of Science:
- Immunogenetics
- Molecular Biology
- Allergy Research
Background:
- Interleukin-18 (IL-18) exhibits dual roles in allergic responses.
- Genetic variations within the IL-18 gene's coding regions have not been previously documented.
Purpose of the Study:
- To identify genetic polymorphisms in the coding regions of the IL-18 gene.
- To examine the association between identified IL-18 gene polymorphisms and asthma in a case-control study.
Main Methods:
- Screening of IL-18 gene coding regions for polymorphisms using PCR-single-stranded conformation polymorphism.
- Direct sequencing of PCR products to confirm polymorphisms.
- Case-control analysis to assess the association between polymorphisms and asthma.
Main Results:
- A single polymorphism, 105A/C, was identified in the IL-18 coding region.
- The 105A allele was significantly more prevalent in asthma patients compared to controls (OR=1.83, P<0.01).
- Significant linkage disequilibrium was noted between the 105A/C and 5' flanking region (-137G/C) polymorphisms.
Conclusions:
- The 105A/C polymorphism in the IL-18 gene is a potential risk factor for asthma.
- This genetic variation may contribute to the pathogenesis of asthma.
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