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RNA expression profiles and data mining of sugarcane response to low temperature
Fábio T S Nogueira1, Vicente E De Rosa, Marcelo Menossi
1Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas, 13083-970, Campinas, SP, Brazil.
Plant Physiology
|August 13, 2003
Summary
Sugarcane (Saccharum sp.) exhibits cold-inducible gene expression, with 20 novel genes identified. Researchers propose a molecular model for sugarcane
Area of Science:
- Plant Science
- Molecular Biology
- Genomics
Background:
- Tropical and subtropical plants are sensitive to cold stress.
- Understanding sugarcane's cold response is crucial for crop resilience.
Purpose of the Study:
- To analyze gene expression profiles in sugarcane under cold stress.
- To identify novel cold-inducible and cold-repressed genes in sugarcane.
- To develop a molecular model for sugarcane's response to low temperatures.
Main Methods:
- Utilized nylon filter arrays to analyze 1,536 expressed sequence tags (ESTs) from sugarcane.
- Investigated gene expression changes over 3 to 48 hours of cold exposure.
- Performed data mining on the Sugarcane EST project database (SUCEST) for homologous proteins.
Main Results:
- Identified 34 cold-inducible ESTs, including 20 novel cold-responsive genes.
- Discovered genes such as cellulose synthase and phosphate transporter were cold-inducible.
- Found 25 ESTs were down-regulated, and identified 33 homologous putative cold-regulated proteins in SUCEST.
Conclusions:
- Significant cold-inducible gene expression occurs in sugarcane.
- Novel cold-responsive genes were identified, expanding knowledge of plant cold stress mechanisms.
- A molecular model for sugarcane's response to low temperature was proposed based on expression profiles and data mining.