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Preparing and Injecting Embryos of Culex Mosquitoes to Generate Null Mutations using CRISPR/Cas9
Published on: September 10, 2020
[Studies on the life cycle of Culex pipiens pallens in Korea]
Duck Sang Lee1, Hak Kyoung Yoon, Hyo Soon Kim
1Department of Biology, College of Liberal Arts and Sciences, Kyung-Hee University, Seoul, Korea.
Abstract:
The present studies were carried out, under natural room condition from June to July in 1969. 1. The period required for growth of eggs and larva varied according to various temperatures and methods of rearing. 2. The average number of eggs laid by the Culex pipiens pallens was 137 per egg raft. 3. The period of gonotrophic cycle was from 3 to 6 days: incubation period of eggs was from 1.5 to 2 days; larva period was from 10 to 14 days: pupa period was from 2 to 3 days. Accordingly, the period from the beginning of gonotrophic cycle to the adult mosquitos in next generation required 25 days at the longest and 16.5 days at the shortest.

