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[Determination of trace copper based on the fluorescence quenching of phenanthroline]
1College of Chemistry and Chemical Engineering, Inner Mongolia University, Hohhot 010021, China.
Abstract:
A fluorescence quenching method for the determination of trace Cu (II) was developed. The method was based on the fluorescence quenching effect of phenanthroline due to the formation of a non-fluorescent complex in pH 6.25-7.55 KH2PO4-Na2HPO4 medium. The excitation and emission wavelengths are 266 and 368 nm, respectively. The linear range is 8-300 ng.mL-1 and the detection limit is 10.4 ng.mL-1. The method has been satisfactorily used to determine trace amounts of Cu (II) in standard environmental water samples with sulfhydryl cotton separation.
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