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Updated: Sep 20, 2026

Practical Aspects of Sample Preparation and Setup of 1H R1ρ Relaxation Dispersion Experiments of RNA
Published on: July 9, 2021
Accurate determination of small one-bond heteronuclear residual dipolar couplings by F1 coupled HSQC modified with a
Krisztina Fehér1, Stefan Berger, Katalin E Kövér
1Institute for Analytical Chemistry, University of Leipzig, Johnnisallee 29, D-04103, Leipzig, Germany.
Abstract:
We report a G-BIRD(r) modified coupled HSQC experiment for the accurate determination of one-bond heteronuclear residual dipolar couplings. The G-BIRD(r) module has been employed to refocus the long-range coupling evolution of the heteronucleus during the t1 frequency labeling period. As a result, the crosspeaks obtained are split by only the direct one-bond coupling that can be extracted by measuring simple frequency differences between singlet maxima. Additionally the decoupling of long-range multiple bond splittings leads to considerable sensitivity enhancement. The modification also has been applied in a TROSY sequence resulting in a significant sensitivity and resolution improvement.
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