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Size exclusion chromatography on soft and semi-rigid packing materials in the dynamic axial compression mode
M B Baru1, A V Danilov, I V Vagenina
1Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow Region 142290, Puschino, Russian Federation. synchro@fibkh.serpukhov.su
Abstract:
The effect of dynamic axial compression within a range of up to 5 bar upon the structure of the bed packed with soft and semi-rigid packing materials (Sephadex G-25, Bio-Gel P2 and Toyopearl HW-40) and the associated chromatographic parameters were studied for size exclusion chromatography. Continuous packing compression is accomplished by use of a special column with controlled external pressure applied to the packing. Compression has been shown to favor an overall increase in the resolution with pressure optima observed in some cases.