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A practical high-throughput screening system for enantioselectivity by using FTIR spectroscopy.

Patrick Tielmann1, Matthias Boese, Martin Luft

  • 1Max-Planck-Institut für Kohlenforschung Kaiser-Wilhelm-Platz 1, 45470 Mülheim/Ruhr, Germany.

Chemistry (Weinheim an Der Bergstrasse, Germany)
|August 14, 2003
PubMed
Summary

Fourier-transform infrared (FTIR) spectroscopy now measures enantiomeric purity using labeled pseudo-enantiomers. This method offers high throughput for applications in catalysis and enzyme evolution.

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Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Chiral Chemistry

Background:

  • Enantiomeric purity is crucial in pharmaceuticals and asymmetric synthesis.
  • Existing methods for determining enantiomeric purity can be time-consuming or require specialized equipment.

Purpose of the Study:

  • To introduce a novel method for measuring enantiomeric purity using Fourier-transform infrared (FTIR) spectroscopy.
  • To demonstrate the application of FTIR spectroscopy with isotopically labeled pseudo-enantiomers for accurate enantiomeric excess (ee) determination.

Main Methods:

  • Utilized (13)C-labeled pseudo-enantiomers to create distinct spectral signals.
  • Applied Lambert-Beer's law to quantify the concentrations of individual enantiomers.
  • Employed a high-throughput FTIR system for rapid sample analysis.

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Main Results:

  • Achieved an accuracy of +/-5% for enantiomeric excess (ee) values with the developed method.
  • Demonstrated a throughput of up to 10,000 samples per day using a commercial high-throughput FTIR system, with a slightly reduced accuracy of +/-7% for ee values.
  • Validated the feasibility of FTIR spectroscopy for enantiomeric purity assessment.

Conclusions:

  • FTIR spectroscopy provides a viable and high-throughput method for determining enantiomeric purity.
  • The technique is particularly relevant for combinatorial symmetric catalysis and directed evolution of enantioselective enzymes.
  • This approach offers a new tool for chiral analysis in chemical and biochemical research.