Related Experiment Video
Updated: Jul 1, 2026

Medium Preparation for the Cultivation of Microorganisms under Strictly Anaerobic/Anoxic Conditions
Published on: August 15, 2019
[Metabolism of rumen microbial populations formed on biosubstrates with different assimilation under the effect of
L H Kalachniuk1, O Ie Vozna, H I Kalachniuk
1Biotechnology Research Institute of Animal Production Gzhytskyi Lviv Academy of Veterinary Medicine, Ukraine.
Abstract:
It has been established that metabolism of mixed microbial population formed on easy assimilated sources of energy and nitrogen (concentrate diet) progressed on higher level. There is increase of amilolytic activity, formation of lactate, ammonia, low molecular carbonic acids with predomination of propionate molar fraction. The increased resistance to effect of pentachlorophenol (PCP) is characteristic nature of the latter. The role of the most resistant synthrophic bacteria to PCP increases. The pure strains of Streptococcus bovis and Megasphaera elsdenii do not stop metabolism at 100 microM of PCP. Mixed population of microorganisms formed on hard accessible biosubstrates (cellulose) and Butyrivibrio fibrisolvens have the increased cellulosolytic activity and while the high sensibility even to low doses of PCP (10-40 microM) is observed. It has been supposed that mechanism of PCP effect is ambiguous for various species of microbial complex of rumen. It's effect strength on all main chains of metabolism (membrane transport, energetic exchange, protein biosynthesis, etc.) significantly depends on capacity of pool of metabolic intermediates formed as a result of definite program of biotechnology of nurture, but significantly decreases the harmful effect of biocides.
Related Concept Videos
Metabolism of Chemolithotrophs
Microbial Mats
Microbes and Methanogenesis
Microbial Bioremediation of Hydrocarbons
Microbial Bioremediation of Pesticides
Bioplastics

