Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

OcaB regulates transitional B cell selection.

Mila Jankovic1, Michel C Nussenzweig

  • 1Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10021-6399, USA.

International Immunology
|August 15, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A designed overlapping variant immunogen pool elicits broad sarbecovirus neutralization.

bioRxiv : the preprint server for biology·2026
Same author

Time to HIV rebound after infusion of long-acting broadly neutralising antibodies 3BNC117-LS and 10-1074-LS and analytical treatment interruption (the RIO trial): a double-blind, randomised, placebo-controlled trial.

The lancet. HIV·2026
Same author

Protocol for an in vivo CRISPR screen for germinal center B cells in mice using ecotropic retrovirus.

STAR protocols·2026
Same author

Diverse paths to broadly neutralizing antibody escape among HIV-1 strains.

Nature microbiology·2026
Same author

Induction of broadly neutralizing HIV antibodies by a two-step mechanism informs vaccine design.

Science (New York, N.Y.)·2026
Same author

Antibody-mediated diversification of primary and secondary humoral immune responses.

The Journal of experimental medicine·2026
Same journal

Thymic cortical epithelial Psmb11-encoded β5t in mouse and human.

International immunology·2026
Same journal

Signaling and Transcriptional Control of Thymocyte Differentiation.

International immunology·2026
Same journal

17,18-epoxyeicosatetraenoic acid and its metabolite attenuate IL-33-induced airway inflammation involving group 2 innate lymphoid cells.

International immunology·2026
Same journal

FcμR enhances recall responses by promoting the generation of CD80+PD-L2+ memory B cells.

International immunology·2026
Same journal

Mitochondrial Transfer-Driven Immune Evasion in the Tumor Microenvironment.

International immunology·2026
Same journal

Granulocyte heterogeneity in immune-mediated and inflammatory diseases: Insights from single-cell transcriptomic analyses.

International immunology·2026
See all related articles

OcaB (Obf-1) is crucial for B cell development and antibody repertoire selection. Mice lacking OcaB show abnormal B cell maturation and a skewed antibody repertoire, indicating its essential role in immune system development.

Area of Science:

  • Immunology
  • Molecular Biology
  • Genetics

Background:

  • OcaB (Obf-1) is a transcriptional co-activator regulating Igkappa gene expression.
  • It plays a vital role in transitional B cell development and germinal center formation.
  • OcaB deficiency leads to abnormal B cell development and a skewed Igkappa repertoire.

Purpose of the Study:

  • To investigate the role of OcaB in B cell receptor (BCR)-mediated B cell selection.
  • To determine if OcaB is essential for antibody repertoire selection during B cell development.

Main Methods:

  • Utilized OcaB knockout (OcaB(-/-)) mice.
  • Introduced a pre-recombined alpha hen egg lysozyme (HEL) Ig transgene into OcaB(-/-) mice.
  • Analyzed B cell development stages and antibody repertoire.

Related Experiment Videos

Main Results:

  • OcaB(-/-) mice exhibit abnormal B cell development, progressing normally to the immature stage but failing to reach the transitional stage.
  • The absence of OcaB results in a skewed Igkappa repertoire, including the presence of anti-DNA antibodies.
  • BCR-mediated selection is impaired in OcaB-deficient developing B cells.

Conclusions:

  • OcaB is indispensable for normal B cell development and the selection of a diverse antibody repertoire.
  • The study highlights OcaB's critical function in preventing the development of potentially self-reactive B cells.
  • OcaB is essential for maintaining immune tolerance through proper antibody repertoire selection.