Related Experiment Video

Updated: Sep 20, 2026

Investigation of Genetic Dependencies Using CRISPR-Cas9-based Competition Assays
11:05

Investigation of Genetic Dependencies Using CRISPR-Cas9-based Competition Assays

Published on: January 7, 2019

SUV39H1 interacts with AML1 and abrogates AML1 transactivity. AML1 is methylated in vivo

Soumen Chakraborty1, Kislay Kumar Sinha, Vitalyi Senyuk

  • 1Department of Pathology and The Cancer Center, University of Illinois at Chicago, Chicago, IL 60607, USA.

Oncogene
|August 15, 2003
PubMed

Insights

The histone methyltransferase SUV39H1 interacts with Acute Myeloid Leukemia 1 (AML1), a key gene regulator. This interaction inhibits AML1

Area of Science:

  • Molecular Biology
  • Epigenetics
  • Hematopoiesis

Background:

  • Acute Myeloid Leukemia 1 (AML1) is a crucial DNA-binding protein regulating hematopoietic gene expression.
  • AML1 is essential for fetal liver hematopoiesis in mice.
  • Previous studies show AML1 interacts with histone acetyltransferases and deacetylases.

Purpose of the Study:

  • To investigate the interaction between AML1 and histone methyltransferase SUV39H1.
  • To understand how SUV39H1 affects AML1's function in gene regulation.

Main Methods:

  • Co-immunoprecipitation assays to detect protein complexes.
  • Analysis of AML1 N-terminus and Runt domain involvement.
  • Assessment of AML1's DNA-binding and transactivating properties.

Main Results:

  • SUV39H1 forms a complex with AML1.
  • The interaction requires AML1's N-terminus, including the Runt domain.
  • SUV39H1 binding abrogates AML1's DNA-binding and transactivating capabilities.
  • AML1's nuclear structure is disrupted upon binding to SUV39H1.

Conclusions:

  • SUV39H1 negatively regulates AML1 activity.
  • Histone methyltransferases are involved in modulating AML1's gene regulatory functions.
  • This interaction provides new insights into epigenetic control of hematopoiesis.

More Related Videos

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images09:42

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Published on: September 7, 2017

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying DNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
Epigenetic Regulation01:37

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
Epigenetic Regulation01:46

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.