Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

MDM2-ARF complex regulates p53 sumoylation.

Lihong Chen1, Jiandong Chen

  • 1Molecular Oncology Program, H Lee Moffitt Comprehensive Cancer Center and Research Institute, 12902 Magnolia Drive, Tampa, FL 33612, USA.

Oncogene
|August 15, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dietary N-Carbamylglutamate Partially Alleviates High-Starch-Induced Hepatic Oxidative Stress and Glycogenic Hepatopathy in Largemouth Bass (<i>Micropterus salmoides</i>).

Antioxidants (Basel, Switzerland)·2026
Same author

Charge transport and mode transition in dual-energy electron beam diodes.

Physical review. E·2026
Same author

Development and Validation of a Prenatal Prediction Model for Neonatal Hyperbilirubinemia Based on Maternal Factors: Revealing Heterogeneous Risk Profiles Across Gestational Age Subgroups.

International journal of women's health·2026
Same author

Field-Emission-Induced Terahertz Plasma Waves and Instabilities in Microdischarges.

Physical review letters·2026
Same author

Scaling law for the space charge oscillation in electron emission diodes and its scale-invariant nature.

Physical review. E·2026
Same author

MDM2 Amplification Enables Selective PROTAC Targeting of Tumor Cells.

Molecular cancer therapeutics·2026
Same journal

DNA methylation-mediated silencing of STAT5A drives breast cancer metastasis via dual regulation of EMT and immunosuppressive microenvironment.

Oncogene·2026
Same journal

LncRNA IRENA promotes peripheral T-cell lymphoma progression through scaffolding ARHGEF1 and FMNL1 to modulate RHOA GTPase/MAPK signaling.

Oncogene·2026
Same journal

A novel circSLIT2-encoded SLIT2 isoform suppresses neural invasion in gastric cancer.

Oncogene·2026
Same journal

The miR-302 family suppresses tumor growth in tongue squamous cell carcinoma by directly targeting P65.

Oncogene·2026
Same journal

MOV10 suppresses circRNA biogenesis by disrupting reverse complementary matches structure to drive hepatocarcinogenesis.

Oncogene·2026
Same journal

HSP47 is a potential dual cell target and prognostic factor in pancreatic cancer.

Oncogene·2026
See all related articles

The p53 tumor suppressor protein

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Cancer Research

Background:

  • The p53 tumor suppressor is regulated by MDM2-mediated ubiquitination and degradation.
  • ARF inhibits MDM2's E3 ligase function, impacting p53 ubiquitination.
  • p53 undergoes modification via SUMO-1 conjugation.

Purpose of the Study:

  • To investigate the regulation of p53 sumoylation.
  • To determine the roles of MDM2 and ARF in p53 sumoylation.
  • To explore the influence of nucleolar targeting on p53 sumoylation.

Main Methods:

  • Analysis of p53 mutants deficient for MDM2 binding.
  • Overexpression studies of MDM2 and ARF.
  • Investigation of ARF's conserved region (102-116) and its role in nucleolar targeting.

Related Experiment Videos

  • Utilizing an MDM2 deletion mutant with an activated nucleolar localization signal.

    • Main Results:

    • A p53 mutant lacking MDM2 binding showed reduced sumoylation.
    • MDM2 overexpression increased p53 sumoylation, further enhanced by ARF.
    • ARF's exon 2 region and nucleolar targeting capability were crucial for stimulating p53 sumoylation.
    • An engineered MDM2 mutant promoted p53 sumoylation via nucleolar targeting independently of ARF.

    Conclusions:

    • p53 sumoylation is regulated by MDM2 and ARF.
    • Nucleolar targeting of p53, mediated by MDM2 and ARF, enhances its sumoylation.
    • This study elucidates a novel mechanism controlling p53 post-translational modification.