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Related Experiment Videos

Acylation stimulating protein stimulates insulin secretion.

B Ahrén1, P J Havel, G Pacini

  • 1Department of Medicine, Lund University, Lund, Sweden. Bo.Ahren@med.lu.se

International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity
|August 15, 2003
PubMed
Summary

Acylation stimulating protein (ASP) enhances glucose-stimulated insulin secretion from pancreatic beta cells. This hormone

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Area of Science:

  • Endocrinology
  • Metabolism
  • Cell Biology

Background:

  • Acylation stimulating protein (ASP) is an adipocyte-derived hormone crucial for fat energy storage.
  • The role of ASP in pancreatic islet function, particularly insulin secretion, remains largely unexplored.

Purpose of the Study:

  • To investigate the effects of Acylation stimulating protein (ASP) on insulin secretion from pancreatic beta cells.
  • To elucidate the mechanisms underlying ASP-mediated insulin secretion and its in vivo effects.

Main Methods:

  • Utilized clonal INS-1 cells and isolated mouse islets to assess insulin secretion in response to glucose and other stimuli.
  • Investigated the involvement of glucose phosphorylation, calcium signaling, and protein kinase C (PKC) in ASP's effects.
  • Administered ASP intravenously to C57BL/6J mice to evaluate its impact on glucose tolerance and insulin response.

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Main Results:

  • ASP dose-dependently augmented glucose-stimulated insulin secretion in INS-1 cells and mouse islets.
  • ASP's effect on insulin secretion was glucose-dependent and involved glucose phosphorylation, calcium uptake, and PKC activation.
  • In vivo, ASP administration enhanced acute insulin response to glucose and improved glucose disposal without altering insulin sensitivity.

Conclusions:

  • Acylation stimulating protein (ASP) directly stimulates insulin secretion from pancreatic beta cells.
  • The mechanism involves glucose phosphorylation, calcium influx, and protein kinase C signaling pathways.
  • ASP enhances glucose tolerance by augmenting insulin secretion and improving glucose disposal in vivo.