[Expression of mitogen-activated protein kinase in vascular tissues after coronary artery balloon injury in rat]
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Updated: Aug 23, 2026
Vascular Balloon Injury and Intraluminal Administration in Rat Carotid Artery
Published on: December 23, 2014
Hua Ge1, Ding-Yin Zeng, Wei-hua Zhao
1Department of Cardiology, The First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning, China.
Objective:
To observe the changes in mitogen-activated protein kinase (MAPK) activity and gene expression after coronary artery balloon injury in rat.
Methods:
Forty Wistar rats were randomly divided into control group (without coronary artery balloon injury), and 3, 7 and 14 days after coronary artery balloon injury groups (n=10, respectively). The activity of MAPK was measured by biochemical method and the gene expression was examined by reverse transcription-polymerase chain reaction (RT-PCR).
Results:
MAPK activity and gene expression of MAPK in the coronary artery balloon injury in rat after 3, 7 and 14 days were significantly higher than that of normal control, especially 3 days after injury [MAPK activity: (17.32+/-2.17) pmol x mg(-1) x min(-1);MAPK protein: ERK1 was 194.7+/-8.6, ERK2 was 175.8+/-7.9; MAPK gene expression: ERK1 was 1.15+/-0.21, ERK2 was 1.13+/-0.14 ].
Conclusion:
The increase activity and mRNA of MAPK may be involved in the restenosis process after coronary balloon injury.
