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Microdissection of Black Widow Spider Silk-producing Glands
Published on: January 11, 2011
Genome sizes of spiders
1Department of Zoology, University of Guelph, Guelph, ON N1G 2W1 Canada. rgregory@genomesize.com
The Journal of Heredity
|August 16, 2003
Summary
This study provides the first genome size estimates for 115 spider species, significantly expanding arachnid data. While no clear patterns emerged, it opens new research avenues for spider genomics.
Area of Science:
- * Zoology
- * Genomics
- * Arachnology
Background:
- * Spiders (Araneomorphae) are a diverse animal group, yet their genome sizes remain largely unstudied.
- * Arachnids are underrepresented in genomic databases, with only one tick species currently documented.
- * Existing data on animal genome sizes lacks comprehensive information on spiders.
Purpose of the Study:
- * To provide novel genome size estimates for a large number of spider species.
- * To expand the genomic data available for the infraorder Araneomorphae.
- * To initiate the exploration of genome size variation within spiders.
Main Methods:
- * Genome size estimation was performed for 115 distinct spider species.
- * The study encompassed spiders from 19 different families within the Araneomorphae infraorder.
- * Flow cytometry or similar quantitative methods were likely employed for genome size determination.
Main Results:
- * New genome size data was generated for 115 spider species, representing 19 families.
- * This substantial dataset considerably increases the available genomic information for spiders.
- * The preliminary survey did not reveal distinct patterns in genome size variation across the studied species.
Conclusions:
- * The study successfully established genome size estimates for a significant number of spider species.
- * The findings highlight the need for further research into spider genome size variation.
- * This work lays the groundwork for future investigations into the evolutionary and ecological factors influencing spider genomes.
Related Concept Videos
Genomic DNA in Eukaryotes
Eukaryotes have large genomes compared to prokaryotes. To fit their genomes into a cell, eukaryotic DNA is packaged extraordinarily tightly inside the nucleus. To achieve this, DNA is tightly wound around proteins called histones, which are packaged into nucleosomes that are joined by linker DNA and coil into chromatin fibers. Additional fibrous proteins further compact the chromatin, which is recognizable as chromosomes during certain phases of cell division.
DNA Packaging
Overview
Genome Size and the Evolution of New Genes
While every living organism has a genome of some kind (be it RNA, or DNA), there is considerable variation in the sizes of these blueprints. One major factor that impacts genome size is whether the organism is prokaryotic or eukaryotic. In prokaryotes, the genome contains little to no non-coding sequence, such that genes are tightly clustered in groups or operons sequentially along the chromosome. Conversely, the genes in eukaryotes are punctuated by long stretches of non-coding sequence.
Chromatin Packaging
Each human somatic cell contains 6 billion base-pairs of DNA. Each base-pair is 0.34 nm long, which means that each diploid cell contains a staggering 2 meters of DNA. How is such a long DNA strand packed inside a nucleus measuring only 10 - 20 microns in diameter?
The chromatin
In combination with specialized DNA binding protein called Histones, the DNA double helix forms a compact DNA: protein complex called chromatin. The chromatin itself is further compacted into higher-order structures.
The chromatin
In combination with specialized DNA binding protein called Histones, the DNA double helix forms a compact DNA: protein complex called chromatin. The chromatin itself is further compacted into higher-order structures.
Lampbrush Chromosomes
In 1882, Flemming observed lampbrush chromosomes (LBC) in salamander eggs. Later in 1892, Rückert observed LBCs in shark egg cells and coined the term "lampbrush chromosomes" because they looked like brushes used to clean kerosene lamps.
LBCs are made up of two pairs of conjugating homologous chromatids. Each chromatid consists of alternatively positioned regions of condensed-inactive chromatin and loosely placed-active side loops, which can be contracted and extended. The loops resemble the...
LBCs are made up of two pairs of conjugating homologous chromatids. Each chromatid consists of alternatively positioned regions of condensed-inactive chromatin and loosely placed-active side loops, which can be contracted and extended. The loops resemble the...
Genome Size and the Evolution of New Genes
While every living organism has a genome of some kind (be it RNA, or DNA), there is considerable variation in the sizes of these blueprints. One major factor that impacts genome size is whether the organism is prokaryotic or eukaryotic. In prokaryotes, the genome contains little to no non-coding sequence, such that genes are tightly clustered in groups or operons sequentially along the chromosome. Conversely, the genes in eukaryotes are punctuated by long stretches of non-coding sequence.

