Related Experiment Videos
Non-aerosol detection of explosives with a continuous flow immunosensor
Lisa C Shriver-Lake1, Paul T Charles, Anne W Kusterbeck
1Code 6900, Naval Research Laboratory, 4555 Overlook Ave SW, 20375, Washington, DC, USA. lcs@cbmse.nrl.navy.mil
Analytical and Bioanalytical Chemistry
|August 16, 2003
Summary
A novel biosensor rapidly detects explosives like TNT and RDX in environmental samples. This technology offers faster field screening than traditional lab methods, aiding in site remediation and unexploded ordnance detection.
Area of Science:
- Environmental Science
- Analytical Chemistry
- Biotechnology
Background:
- Explosive contamination (TNT, RDX) poses a global environmental threat.
- Current detection methods involve lengthy off-site laboratory analysis.
- Accurate, rapid measurement is crucial for site assessment and remediation.
Purpose of the Study:
- To review current explosive detection methods.
- To introduce a novel continuous flow biosensor for rapid field screening.
- To enable accurate quantification of TNT and RDX in environmental samples.
Main Methods:
- Development of a continuous flow biosensor utilizing monoclonal antibodies.
- Immobilization of antibodies on solid substrates for displacement immunoassay.
- Detection based on the displacement of fluorescently labeled antigens by explosive compounds.
Main Results:
- The biosensor accurately detects TNT and RDX at ppb to ppt levels.
- The system is effective in groundwater, seawater, and soil extracts.
- Provides rapid field screening, reducing analysis time compared to traditional methods.
Conclusions:
- The developed biosensor offers a rapid and accurate solution for explosive detection.
- Applications include environmental monitoring at remediation sites.
- Potential use in locating underwater unexploded ordnance.