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Related Experiment Videos

Acidification and protein traffic.

Ora A Weisz1

  • 1Renal-Electrolyte Division, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA.

International Review of Cytology
|August 19, 2003
PubMed
Summary
This summary is machine-generated.

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Organelle acidification is crucial for cellular functions and transport. This review explores how pH regulation impacts membrane traffic in mammalian cells, highlighting key mechanisms and future research directions.

Area of Science:

  • Cell Biology
  • Biochemistry
  • Physiology

Background:

  • Organelle acidification, including the Golgi complex, lysosomes, and vesicles, is vital for biochemical functions.
  • Acidic pH is essential for efficient protein and lipid sorting and trafficking in cellular pathways.
  • Understanding pH's role in membrane traffic is limited by diverse methodologies and challenges in isolating transport steps.

Purpose of the Study:

  • To review evidence on the role of acidification in biosynthetic and endocytic transport in mammalian cells.
  • To describe mechanisms regulating and maintaining organelle pH.
  • To discuss methods for monitoring and quantifying organelle pH.

Main Methods:

  • Literature review of old and recent evidence.
  • Discussion of organelle pH regulation mechanisms.

Related Experiment Videos

  • Overview of pH monitoring and quantitation techniques.

    • Main Results:

    • Acidification plays a significant role at various stages of biosynthetic and endocytic transport.
    • Mechanisms for organelle pH regulation and maintenance are diverse.
    • Methods exist to monitor and quantify organelle pH, though challenges remain.

    Conclusions:

    • Organelle pH is a critical regulator of membrane traffic.
    • Further research is needed to fully elucidate the complex interplay between pH and cellular transport.
    • General principles of pH modulation in membrane traffic are emerging.