[Location of the fertility restorer gene for T-type CMS wheat by microsatellite marker]

Through the genetic analysis of a F2 population, derived from CMS line 75-3369A (T-type CMS wheat) and the restorer line 7269-10, the result indicated that the restorer line was conditioned by two dominant genes. A F2 population was used to map the fertility restorer (Rf) gene by microsatellite and BSA (bulked segregant analysis). Restorer and sterile DNA pools were established using the extreme fertile and sterile plants of F2 population, respectively. Among the 230 pairs of microsatellite primers, two markers were found polymorphic between the two pools. Linkage analysis showed that microsatellite marker Xgwm136 and Xgwm550 were linked with the two fertility restorer genes, respectively. One of the Rf gene was located on 1AS and the genetic distance between the SSR marker Xgwm136 and this Rf gene was 6.7 cM, the other Rf gene was located on 1BS and with a genetic distance of 5.1 cM to marker Xgwm550.