Related Experiment Video
Updated: Jul 22, 2026
07:33
In vivo Dual Substrate Bioluminescent Imaging
Published on: October 11, 2011
A new ultrasensitive bioluminogenic enzyme substrate for beta-galactosidase
R Geiger1, E Schneider, K Wallenfels
1Medor GmbH, Herrsching, Germany.
Biological Chemistry Hoppe-Seyler
|December 1, 1992
Abstract:
A derivative of D-luciferin, D-luciferin-O-beta-galactoside, was synthesized and used as highly sensitive substrate for beta-galactosidase. The substrate was physicochemically characterized. Enzymatic cleavage of the new compound by beta-galactosidase was demonstrated and kinetic constants Km, Vmax, kcat and kcat/Km have been determined. The compound has been proved to be a highly sensitive substrate for beta-galactosidase, permitting a limit of detection of 3.7 x 10(-19) mol of enzyme per assay.
