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Fluorescent fibre-optic immunosensing system based on complement lysis of liposome containing carboxyfluorescein
Y Tatsu1, S Yamamura, S Yoshikawa
1Government Industrial Research Institute, Osaka, Japan.
Biosensors & Bioelectronics
|January 1, 1992
Abstract:
A novel type of fibre-optic immunosensing system has been developed. Agar gel-immobilized liposomes containing carboxyfluorescein were attached to the tip of an optical fibre. Complement-mediated immunolysis of the liposome was fluorometrically detected through the fibre. By using dinitrophenyl (DNP) hapten-loaded liposomes, concentrations as low as 500-fold diluted anti-DNP antibody and 0.76 CH50 ml-1 of complement were detected.