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Genotype and interleukin-10 responses after cardiopulmonary bypass
H F Galley1, P R Lowe, R L Carmichael
1Academic Unit of Anaesthesia and Intensive Care, Institute of Medical Sciences, University of Aberdeen, Aberdeen AB25 2ZD, Scotland, UK. h.f.galley@abdn.ac.uk
British Journal of Anaesthesia
|August 20, 2003
Summary
Interleukin-10 (IL-10) levels increase after cardiopulmonary bypass. Higher IL-10 concentrations correlate with increased organ dysfunction, influenced by IL-10 gene variations.
Area of Science:
- Cardiovascular Surgery
- Immunology
- Genetics
Background:
- Cardiopulmonary bypass triggers a significant inflammatory response.
- Interleukin-10 (IL-10) is a key anti-inflammatory cytokine.
- Individual variations in IL-10 levels and genetic predisposition are observed.
Purpose of the Study:
- Investigate the relationship between IL-10 genotype and circulating IL-10 concentrations.
- Determine the association between IL-10 levels and organ dysfunction post-cardiopulmonary bypass.
Main Methods:
- Collected blood samples from 150 patients before, and 3, and 24 hours after cardiopulmonary bypass.
- Measured IL-10 concentrations using enzyme immunoassay.
- Analyzed the IL-10 gene -1082 single nucleotide polymorphism via restriction fragment length polymorphism.
Main Results:
- IL-10 concentrations significantly increased at 3 and 24 hours post-bypass (P<0.0001).
- The G allele at -1082 bp was linked to lower maximal IL-10 levels compared to the A allele (P=0.042).
- Patients with organ dysfunction exhibited higher IL-10 levels at 3 hours post-surgery (P=0.001).
Conclusions:
- The G allele of the IL-10 gene -1082 SNP is associated with reduced IL-10 release after cardiopulmonary bypass.
- Elevated IL-10 secretion post-surgery is linked to increased organ dysfunction within 24 hours.