Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Spatial and temporal changes in Bax subcellular localization during anoikis.

Anthony J Valentijn1, Anthony D Metcalfe, Jane Kott

  • 1Wellcome Trust Centre for Cell Matrix Research, School of Biological Sciences, University of Manchester, 3.35 Stopford Building, Oxford Road, Manchester M13 9PT, UK.

The Journal of Cell Biology
|August 20, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Anti-progestin therapy targets hallmarks of breast cancer risk.

Nature·2025
Same author

Structuring of gellan hydrogel enables the production of inherently antifibrotic, lubricating eye drops.

Acta biomaterialia·2025
Same author

Matrix stiffness drives alterations in aldehyde metabolism, inducing DNA damage and transformation.

Scientific reports·2025
Same author

Corrigendum to "Optimising a self-assembling peptide hydrogel as a Matrigel alternative for 3-dimensional mammary epithelial cell culture" [Biomater. Adv. volume 160, (2024) 213847].

Biomaterials advances·2024
Same author

Trabecular meshwork cell differentiation in response to collagen and TGFβ-2 spatial interactions.

Acta biomaterialia·2024
Same author

Optimising a self-assembling peptide hydrogel as a Matrigel alternative for 3-dimensional mammary epithelial cell culture.

Biomaterials advances·2024

Cell detachment triggers Bax protein translocation to mitochondria, initiating apoptosis. Critical cell fate decisions occur early, before Bax clustering and cytochrome c release, suggesting multi-level Bax regulation.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Bax, a Bcl-2 family protein, is crucial for apoptosis.
  • Bax translocation to mitochondria and oligomerization are key events in releasing apoptogenic factors like cytochrome c.

Purpose of the Study:

  • To investigate the spatial and temporal dynamics of Bax distribution during anoikis.
  • To identify critical time points in the apoptotic pathway regulated by Bax.

Main Methods:

  • Utilized anoikis as a model system to study apoptosis.
  • Examined Bax protein localization and mitochondrial changes in detached cells over time.

Main Results:

  • Bax translocated to mitochondria within 15 minutes of cell detachment.

Related Experiment Videos

  • Mitochondrial permeabilization and Bax clustering occurred hours later, coinciding with caspase activation and loss of mitochondrial membrane potential.
  • Cells could be rescued from anoikis if replated within an hour, indicating a critical window for intervention after Bax translocation but before late apoptotic events.

    • Conclusions:

    • Bax regulation in apoptosis is multi-layered, with early events after translocation being critical for cell fate.
    • Bax oligomerization and cluster formation represent late-stage events in the apoptotic cascade.