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Updated: Sep 20, 2026
Assaying for Inorganic Polyphosphate in Bacteria
Published on: January 21, 2019
Crystallization and preliminary X-ray analysis of inorganic polyphosphate/ATP-glucomannokinase from Arthrobacter sp.
Takako Mukai1, Shigeyuki Kawai, Shigetarou Mori
1Department of Basic and Applied Molecular Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611-0011, Japan.
Abstract:
Inorganic polyphosphate [poly(P)]/ATP-glucomannokinase from Arthrobacter sp. strain KM phosphorylates glucose and mannose, utilizing both ATP and poly(P) as phosphoryl donors. The enzyme was overexpressed in Escherichia coli, purified and crystallized by means of the hanging-drop vapour-diffusion method with ammonium sulfate as the precipitant. The crystals were orthorhombic and belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 66.2, b = 83.7, c = 103.8 A. Assuming two molecules per asymmetric unit, V(sol) is 0.49. X-ray diffraction data to 2.83 A have been collected from a single crystal.
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