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Published on: December 30, 2016
Purification, crystallization and preliminary diffraction studies of the Pseudomonas aeruginosa strain K122-4
Gerald F Audette1, Randall T Irvin, Bart Hazes
1Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta T6G 2H7, Canada.
Abstract:
The monomeric pilin from Pseudomonas aeruginosa strain K122-4 has been crystallized and preliminary X-ray diffraction data have been collected. Pilin is the monomeric subunit of the type IV pilus, the dominant adhesin of the opportunistic pathogen P. aeruginosa. The K122-4 pilin crystallizes as a dimer in space group P1, with unit-cell parameters a = 40.19, b = 38.93, c = 37.22 A, alpha = 66.38, beta = 111.12, gamma = 93.74 degrees. Diffraction data were collected using a synchrotron-radiation source and were processed to 1.54 A d-spacing.
