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Direct determination of single-to-double stranded DNA ratio in solution using steady-state fluorescence measurements
Lori Beach1, Claude Schweitzer, J C Scaiano
1Department of Chemistry, University of Ottawa, 10 Marie Curie, Ottawa, Ontario, Canada K1N 6N5.
Organic & Biomolecular Chemistry
|August 21, 2003
Abstract:
We report a simple and rapid method for quantitation of single-to-double stranded (ss : ds) DNA ratios in solution, using steady-state measurements of fluorescence from two simultaneously excited intercalated dyes; the ratio of fluorescence intensities from PicoGreen (525 nm) and ethidium bromide (610 nm) is directly proportional to the ss : ds DNA ratio.