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Updated: Sep 20, 2026
Determination of Sialic Acids in Liver and Milk Samples of Wild-type and CMAH Knock-out Mice.
Published on: July 14, 2017
Complete isolation and characterization of silybins and isosilybins from milk thistle (Silybum marianum)
Nam-Cheol Kim1, Tyler N Graf, Charles M Sparacino
1Natural Product Laboratory, Chemistry and Life Sciences, Research Triangle Institute, 3040 Cornwallis Road, Research Triangle Park, North Carolina 27709, USA. nck@rti.org, mcw@rti.org
Abstract:
Complete separation, isolation, and structural characterization of four diastereoisomeric flavonolignans, silybins A (1) and B (2), and isosilybins A (3) and B (4) from the seeds of milk thistle (Silybum marianum) were achieved for the first time using a preparative reversed-phase HPLC method. In addition, three other flavonolignans, silychristin (5) isosilychristin (6) and silydianin (7), and a flavonoid, taxifolin (8) were isolated. Structures, including absolute stereochemistries of 1-4, were confirmed using 2D NMR and CD spectroscopy.
