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Updated: Sep 20, 2026

Drug-induced Sensitization of Adenylyl Cyclase: Assay Streamlining and Miniaturization for Small Molecule and siRNA Screening Applications
Published on: January 27, 2014
Desensitization of H2-like histamine receptors stimulating cyclic AMP formation in the chick cerebral cortex
Paulina Sedkowska1, Jolanta B Zawilska, Jerzy Z Nowak
1Department of Biogenic Amines, Polish Academy of Sciences, POB-225, PL 90-950 Lódź, Poland.
Abstract:
Histamine potently stimulates cyclic AMP formation in slices of the chick cerebral cortex. Pretreatment of the tissue slices with 10 microM histamine for 2-30 min led to a time-dependent attenuation (when compared with values observed in the control tissue) of the cyclic AMP response produced by subsequent re-stimulation with 1, 10 or 100 microM histamine. The observed histamine-induced desensitization appears to be specific and homologous as the increase in cyclic AMP formation evoked by both forskolin or pituitary adenylate cyclase-activating polypeptide (PACAP) in slices pretreated with 10 microM histamine for 15 min was unchanged. It is concluded that in the chick cerebral cortex, H2-like receptors linked to cyclic AMP-generating system undergo rapid homologous desensitization.
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