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Updated: Jan 25, 2026

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Published on: February 3, 2023
Linking floc structure and settling properties to activated sludge population dynamics in an SBR
R Govoreanu1, D Seghers, I Nopens
1BIOMATH, Ghent University, Coupure Links 653, Ghent, Belgium.
Activated sludge properties in sequencing batch reactors (SBRs) were studied over 227 days. Changes in microbial communities correlated with floc size and settling properties, indicating distinct operational stages.
Area of Science:
- Environmental Engineering
- Microbiology
- Wastewater Treatment
Background:
- Activated sludge properties are crucial for efficient wastewater treatment.
- Understanding microbial community dynamics and floc characteristics is key to optimizing treatment processes.
- Sequencing batch reactors (SBRs) offer a flexible approach to wastewater treatment.
Purpose of the Study:
- To analyze the properties of activated sludge in an SBR over 227 days.
- To correlate settling properties (sludge volume index) with floc size and microbial community dynamics.
- To identify distinct stages of SBR evolution based on these parameters.
Main Methods:
- On-line laser light scattering for floc size and distribution analysis.
- Denaturing gradient gel electrophoresis (DGGE) of 16S rRNA genes for microbial community analysis.
- Microscopical observations and sludge volume index (SVI) measurements.
Main Results:
- Three distinct stages of SBR operation were observed.
- Stage 1: Predominance of floc-forming bacteria, good correlation between floc size and community.
- Stage 2: Balance of bacteria, good settling, dynamic community.
- Stage 3: Increase in filamentous bacteria, altered floc distribution, new dominant species.
Conclusions:
- Microbial community structure significantly impacts activated sludge floc properties and settling.
- SBR performance is linked to the balance between floc-forming and filamentous bacteria.
- Monitoring floc characteristics and microbial dynamics provides insights into SBR operational stages.
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