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Updated: Dec 28, 2025

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes
Published on: November 1, 2019
Aminoglycoside (neomycin) preference is for A-form nucleic acids, not just RNA: results from a competition dialysis
Dev P Arya1, Liang Xue, Bert Willis
1Laboratory of Medicinal Chemistry, Department of Chemistry, Clemson University, Clemson, South Carolina 29634, USA. dparya@clemson.edu
Abstract:
Aminoglycosides have been at the forefront of antimicrobial therapy for the past 50 years. Their specificity is believed to lie in binding duplex RNAs (rRNA). Competition dialysis studies of various nucleic acid forms with 9-aminoacridine, quinacrine, and a neomycin-acridine conjugate were carried out. Our results suggest a strong preference for aminoglycoside binding to nucleic acids that can adopt an A-type conformation. These results challenge the common belief that aminoglycoside specificity is simply for duplex RNAs.
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