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Updated: Sep 20, 2026
Quantitative In vitro Assay to Measure Neutrophil Adhesion to Activated Primary Human Microvascular Endothelial Cells under Static Conditions
Published on: August 23, 2013
Modulation of neutrophil adhesion to vascular endothelial cells in rat experimental otitis media treated with a
Fuyuki Enomoto1, Ryutaku Kin, Takeshi Kataoka
1Department of Otorhinolaryngology, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyu-ku, Tokyo 113-8421, Japan. fenomoto@med.juntendo.ac.jp
Object:
We previously reported that erythromycin (EM) affected the adhesion molecules that interact with neutrophils and inhibited neutrophil infiltration in a rat experimental otitis media model.
Purpose:
The purpose of the present study was to examine whether EM affects the adhesion of rat neutrophils to endothelial cells.
Methods:
The adhesion of neutrophils to WK-5 endothelial cells was evaluated. One group of rats was given EM for 2 weeks, while the other group received no treatment. The adhesion of peripheral-blood neutrophils and neutrophils accumulated in the middle ear from these two groups were compared.
Results:
Two-week EM pretreatment inhibited adhesion of neutrophils to WK-5 cells. The adhesion of neutrophils accumulated in the middle ear cavity to WK-5 cells was significantly higher than that of peripheral-blood neutrophils from control rats.
Conclusion:
The results suggest that EM affects the adhesion of neutrophils to endothelial cells.
Insights
Erythromycin (EM) pretreatment inhibits neutrophil adhesion to endothelial cells. This study reveals EM
Area of Science:
- Immunology
- Pharmacology
- Otolaryngology
Background:
- Erythromycin (EM) previously demonstrated inhibition of neutrophil infiltration in experimental otitis media.
- Neutrophil adhesion to endothelial cells is a critical step in inflammatory processes.
Purpose of the Study:
- To investigate the effect of erythromycin (EM) on the adhesion of rat neutrophils to endothelial cells.
- To determine if EM influences neutrophil-endothelial cell interactions.
Main Methods:
- Neutrophil adhesion to WK-5 endothelial cells was assessed.
- Rats received either 2-week EM pretreatment or no treatment.
- Adhesion of peripheral blood neutrophils and middle ear-accumulated neutrophils was compared between groups.
Main Results:
- Two-week EM pretreatment significantly inhibited neutrophil adhesion to WK-5 endothelial cells.
- Neutrophils from the middle ear cavity exhibited higher adhesion to WK-5 cells compared to peripheral blood neutrophils from control rats.
Conclusions:
- Erythromycin (EM) demonstrably affects neutrophil adhesion to endothelial cells.
- The findings suggest a mechanism by which EM may modulate inflammatory responses in otitis media.
