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Updated: Sep 20, 2026

A Uniform Shear Assay for Human Platelet and Cell Surface Receptors via Cone-plate Viscometry
Published on: June 5, 2019
Recombinant protein 1/secretoglobin 1A1 participates in the actin polymerization of human platelets
1Department of Laboratory Medicine, Asahikawa Medical College, Asahikawa 078-8510, Japan. yuhayasi@asahikawa-med.ac.jp
Background:
Human protein 1 which was originally isolated from pathological urine is identified with clara cell 10 kDa protein and uteroglobin. It has immunomodulatory and anti-inflammatory effects in many vertebrates. Although there have been thorough studies on the structure, molecular biology and biochemical characteristics, the precise mechanism of its biological activities is unknown. The purpose of this research is to clarify the biochemical mechanism of protein 1 through its effect on the platelet aggregation process.
Methods:
Changes in calcium mobilization, actin filament concentrations and functions of integrin alphaIIbbeta3 resulting from platelet stimulation were measured in the presence or absence of recombinant protein 1 (rP1).
Results:
Recombinant protein 1 inhibited U46619- and thapsigargin-induced platelet aggregation by preventing store mediated calcium entry (SMCE). The binding of rP1 to resting platelets induced an increase in the actin filament that accompanied the structural changes of alphaIIbbeta3. When rP1-pretreated platelets were activated by thrombin or thapsigargin, the alterations in the actin filament and alphaIIbbeta3 resulting from the stimulation decreased.
Conclusion:
These results suggest that rP1 inhibits platelet aggregation by participation in the actin polymerization through which SMCE is mediated.
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