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Recombinant Protein Expression, Crystallization, and Biophysical Studies of a Bacillus-conserved Nucleotide Pyrophosphorylase, BcMazG
Published on: May 16, 2017
Isolation and partial biochemical characterization of a proteinaceous anti-bacteria and anti-yeast compound produced
M Atanassova1, Y Choiset, M Dalgalarrondo
1Department of Microbiology, Faculty of Biology, University of Sofia, Blvd. Dragan Tzankov, No. 8, 1423 Sofia, Bulgaria.
Abstract:
New proteinaceous active substance produced by Lactobacillus paracasei subsp. paracasei strain M3 used as a starter for Bulgarian yellow cheese was identified and studied. It displayed bactericidal and fungistatic activities. Its activity was checked against over 60 bacterial and yeast strains. It was efficient against Bacillus subtilis ATCC 6633, several L. delbrueckii species, Helicobacter pylori NCIPD 230 and some yeast species, for example Candida albicans, C. pseudointermedia NBIMCC 1532, C. blankii NBIMCC 85 and Saccharomyces cerevisiae NBIMCC 1812. The synthesis of the substance by producing strain was detected in the late logarithmic growth phase during batch fermentation. Anion exchange chromatography, reversed phase chromatography (RPC) on C4 column and HPLC on C18 column were used for partial purification of this antimicrobial compound. The gene responsible for the synthesis of the active substance is located on the bacterial chromosome.
Insights
Researchers identified a new antimicrobial substance from Lactobacillus paracasei strain M3. This protein shows bactericidal and fungistatic activity against various pathogens, including Candida albicans and Helicobacter pylori.
Area of Science:
- Microbiology
- Biochemistry
- Food Science
Background:
- Lactobacillus paracasei subsp. paracasei strain M3 is used as a starter culture in Bulgarian yellow cheese production.
- Antimicrobial substances produced by lactic acid bacteria can contribute to food safety and health benefits.
Purpose of the Study:
- To identify and characterize a novel proteinaceous active substance produced by Lactobacillus paracasei strain M3.
- To evaluate the antimicrobial spectrum and potential applications of this substance.
Main Methods:
- Bacterial strain isolation and cultivation.
- Anion exchange chromatography, reversed-phase chromatography (RPC), and high-performance liquid chromatography (HPLC) for partial purification.
- Antimicrobial activity assays against a panel of bacterial and yeast strains.
Main Results:
- A novel protein with bactericidal and fungistatic activities was identified.
- The substance demonstrated efficacy against Bacillus subtilis, Helicobacter pylori, and several Candida and Saccharomyces species.
- Synthesis of the antimicrobial compound was linked to the late logarithmic growth phase.
- The gene responsible for synthesis is located on the bacterial chromosome.
Conclusions:
- Lactobacillus paracasei strain M3 produces a potent antimicrobial protein with a broad spectrum of activity.
- This substance holds potential for applications in food preservation and as a therapeutic agent.
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