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Updated: Aug 1, 2026

Bioindication Testing of Stream Environment Suitability for Young Freshwater Pearl Mussels Using In Situ Exposure Methods
Published on: September 5, 2018
DNA damage in digestive gland and mantle tissue of the mussel Perna perna
Eduardo Alves de Almeida1, Sabrina de Almeida Marques, Clécio Fernando Klitzke
1Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, CP 26.077, Av. Prof. Lineu Prestes 748, São Paulo, SP, CEP 05513-970, Brazil.
Abstract:
Data concerning the susceptibility of DNA to damage by reactive oxygen and nitrogen species and other endogenous compounds produced by physiological stress in marine organisms is lacking, especially in bivalve mollusks. In this article, we analyzed the background levels of lipid peroxidation (malondialdehyde, MDA), 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) and 1,N2-etheno-2'-deoxyguanosine (1,N2-epsilon dGuo) in digestive gland and mantle tissue of mussels Perna perna collected at a cultivation zone in Florianópolis (Santa Catarina, Brazil). The present data point to the possibility of the use of both 8-oxodGuo and 1,N2-epsilon dGuo as complementary indicators of oxidative stress processes in mussels. A sensitive method coupling high performance liquid chromatography to mass spectrometry was applied for the detection of 1,N2-epsilon dGuo in mussel tissues.
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