Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

MHC recognition by hapten-specific HLA-A2-restricted CD8+ CTL.

Susan J Gagnon1, Zichun Wang, Richard Turner

  • 1Molecular Immunology Section, Neuroimmunology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA.

Journal of Immunology (Baltimore, Md. : 1950)
|August 21, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Advanced glucose control strategies leveraging Raman spectroscopy for optimized mammalian cell culture manufacturing.

Biotechnology progress·2026
Same author

Firm, Yellow, and Now Fluid-Filled!

Clinical and experimental dermatology·2026
Same author

Patient and diagnosis delays among migrant tuberculosis patients in suburban Shanghai, China: a 10-year retrospective study.

BMC infectious diseases·2026
Same author

Decoupling Kinetically Coupled Steps via Hierarchical Relay Catalysis on High-Entropy Alloy for Efficient Ammonia Decomposition.

Angewandte Chemie (International ed. in English)·2026
Same author

Systematic Review of Applications of Near-Infrared Spectroscopy in Forest Engineering and Wood Industry: Progress and Perspectives.

Critical reviews in analytical chemistry·2026
Same author

Turning struggles into strengths: A qualitative exploration of academic difficulty in medical school.

Medical teacher·2026

T cell receptors (TCRs) recognize peptide-MHC complexes. This study shows that both peptide-specific and hapten-specific TCRs recognize similar HLA-A2 molecular features, indicating common structural elements are key for TCR ligand recognition.

Area of Science:

  • Immunology
  • Molecular Biology
  • Structural Biology

Background:

  • T cell receptors (TCRs) recognize peptide-antigens presented by Major Histocompatibility Complex (MHC) molecules.
  • Previous studies demonstrated that TCRs specific for different peptides presented by HLA-A2 recognize shared molecular features on the MHC molecule.
  • Hapten-specific T cells often exhibit reduced MHC dependence, prompting investigation into their recognition mechanisms.

Purpose of the Study:

  • To determine if hapten-specific, HLA-A2-restricted T cell receptors (TCRs) recognize the same molecular features on HLA-A2 as peptide-specific TCRs.
  • To investigate the MHC dependence and restriction of TCRs recognizing haptens bound to peptides.

Main Methods:

  • Utilized a panel of CD8+ cytotoxic T lymphocytes (CTLs) specific for the hapten DNP bound to two different peptides presented by HLA-A2.

Related Experiment Videos

  • Assessed TCR recognition of wild-type and mutated HLA-A2 molecules.
  • Analyzed the impact of specific HLA-A2 mutations on T cell recognition in the presence and absence of hapten.

    • Main Results:

    • Hapten-specific CD8+ CTLs demonstrated stringent MHC restriction, similar to peptide-specific CTLs.
    • Recognition by these hapten-specific CTLs was significantly affected by the same HLA-A2 mutations that impacted peptide-specific CTL recognition.
    • A subset of hapten-specific CTLs could recognize a mutated HLA-A2 molecule even without the hapten.

    Conclusions:

    • TCR recognition of diverse ligands, including haptens, presented by HLA-A2 relies heavily on common structural elements within the central peptide-binding site.
    • These findings suggest a conserved mechanism for TCR engagement with peptide-MHC complexes, irrespective of whether the primary recognition involves a peptide or a hapten.