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Related Experiment Videos

A transgenic mouse model genetically tags all activated CD8 T cells.

Charles H Maris1, Joseph D Miller, John D Altman

  • 1Department of Microbiology and Immunology, Emory Vaccine Center, Emory University, Atlanta, GA 30329, USA.

Journal of Immunology (Baltimore, Md. : 1950)
|August 21, 2003
PubMed
Summary

Researchers developed a new transgenic mouse model to track CD8+ T cells. This model permanently marks activated CD8+ T cells, enabling the study of cellular immune responses without prior epitope knowledge.

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Area of Science:

  • Immunology
  • Cellular Biology
  • Virology

Background:

  • Identifying antigen-specific CD8+ T cells is crucial for understanding immunological memory.
  • Current methods like MHC tetramers and cytokine assays require prior knowledge of epitopes and MHC backgrounds.
  • There is a need for alternative strategies to track CD8+ T cell responses universally.

Purpose of the Study:

  • To develop and validate a novel transgenic mouse model for permanently marking activated CD8+ T cells.
  • To assess the utility of this model in tracking antiviral CD8+ T cell dynamics.
  • To demonstrate that the marking strategy is independent of specific antigenic epitopes or MHC backgrounds.

Main Methods:

  • Generation of a transgenic mouse model utilizing Cre-loxP recombination.

Related Experiment Videos

  • Permanent marking of activated CD8+ T cells with beta-galactosidase (beta-gal).
  • Utilizing the lymphocytic choriomeningitis virus (LCMV) infection model to study antiviral T cell responses.

    • Main Results:

    • The transgenic model successfully and permanently marks all activated CD8+ T cells with beta-gal.
    • The model allows for tracking the dynamics of antiviral CD8+ T cell populations post-LCMV infection.
    • All antiviral effector and memory CD8+ T cells were found within the beta-gal-marked population.

    Conclusions:

    • This transgenic mouse model provides a powerful tool for studying CD8+ T cell responses.
    • The beta-gal marking strategy is broadly applicable for tracking CD8+ T cells irrespective of epitope specificity or MHC.
    • This approach facilitates research into immunological memory and antiviral immunity.