Related Experiment Video

Updated: Jul 19, 2026

Genotyping of Plant and Animal Samples without Prior DNA Purification
11:00

Genotyping of Plant and Animal Samples without Prior DNA Purification

Published on: September 24, 2012

PCR-based methods facilitate targeted gene manipulations and cloning procedures

Jürgen Wendland1

  • 1Junior Research Group: Growth-control of Fungal Pathogens, Hans-Knöll Institut für Naturstoff-Forschung, Friedrich-Schiller University Jena, Winzerlaer Strasse 10, 07745, Jena, Germany. juergen.wendland@uni-jena.de

Current Genetics
|August 21, 2003
PubMed
Summary

Efficient gene disruption is crucial for understanding new genes. PCR-based methods and in vivo recombination in yeast and E. coli simplify genetic analysis across diverse species.

More Related Videos

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format08:25

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format

Published on: April 8, 2017

Monitoring GPCR-β-arrestin1/2 Interactions in Real Time Living Systems to Accelerate Drug Discovery08:21

Monitoring GPCR-β-arrestin1/2 Interactions in Real Time Living Systems to Accelerate Drug Discovery

Published on: June 28, 2019

Related Concept Videos

PCR01:32

PCR

Overview
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...