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Updated: Aug 9, 2026
Endotoxin Activity Assay for the Detection of Whole Blood Endotoxemia in Critically Ill Patients
Published on: June 24, 2019
Beneficial effects of apolipoprotein A-I on endotoxemia
Takashi Imai1, Tetsuji Fujita, Yoji Yamazaki
1Department of Surgery, Jikei University School of Medicine, 3-25-8 Nishi-shinbashi, Minato-ku, Tokyo 105-8461, Japan.
Purpose:
Although many studies have shown the beneficial effects of lipoproteins on animals with endotoxemia, little is known about the impact of apolipoprotein A-I (apoA-I) on tumor necrosis factor Alpha (TNF-Alpha) release in response to lipopolysaccharide (LPS). The present study was conducted to determine whether the administration of apoA-I inhibits the release of TNF-Alpha and influences the survival rate of rats with endotoxemia.
Methods:
Forty male Wistar rats were divided randomly into four groups. Rats in the first and second groups were given 1 mg/kg LPS intraperitoneally (i.p.) and blood was collected 1 h later to measure the serum levels of TNF-Alpha. Either 10 mg/kg apoA-I or Tris-buffered saline was injected i.p. and the serum TNF-Alpha levels were measured again 2 h later. Rats in the third and fourth groups were given 5 mg/kg LPS. Following the administration of 10 mg/kg apoA-I or Tris-buffered saline, animals were observed for 5 days and survival rates were determined.
Results:
ApoA-I inhibited the release of serum TNF-Alpha and improved the survival rates of rats with endotoxemia.
Conclusion:
The administration of apoA-I suppressed the TNF-Alpha release in endotoxemia and decreased the mortality rates of rats.
