Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A two-dimensional proteome map of Shigella flexneri.

Xiang Liao1, Tianyi Ying, Hengliang Wang

  • 1Institute of Biotechnology, 20 Dongdajie Street, Fengtai District, Beijing 100071, PR China.

Electrophoresis
|August 21, 2003
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Host-independent metagenomics reveal gut bacteria contribution to Delia antiqua growth by vitamin B6 provision.

Insect molecular biology·2026
Same author

Modular Development of a <i>Klebsiella pneumoniae</i> Bioconjugate Nanovaccine Elicits Robust Protection via Intranasal Immunization.

Nanomaterials (Basel, Switzerland)·2026
Same author

Decoding Virulence Mechanisms of <i>Bacillus anthracis</i> Using a <i>Galleria mellonella</i> Infection Model: Differential Host Response Profiles Elicited by AtxA and PlcR.

Microorganisms·2026
Same author

The Dual Role of RsiP in Regulating Virulence and Host Adaptation in <i>Bacillus anthracis</i>.

Pathogens (Basel, Switzerland)·2026
Same author

Efficacy and safety of clevidipine emulsion injection compared with nicardipine in patients with hypertensive emergencies: a randomized, single-blind, positive-parallel-controlled, phase III clinical trial.

Journal of hypertension·2025
Same author

Development of a Promising Ferritin-Based Bioconjugate Nanovaccine Candidate against Brucellosis.

ACS infectious diseases·2025

Researchers mapped proteins of Shigella flexneri, a bacterium causing dysentery. This proteome map aids in developing vaccines and antibacterial drugs against this pathogen.

Area of Science:

  • Microbiology
  • Proteomics
  • Bacterial Pathogenesis

Background:

  • Shigella flexneri is a Gram-negative, facultative intracellular pathogen causing bacillary dysentery.
  • Understanding S. flexneri's protein composition is crucial for developing effective treatments and vaccines.

Purpose of the Study:

  • To create a comprehensive proteome map of Shigella flexneri 2a strain 2457T.
  • To identify extracellular and whole-cell proteins for further research.

Main Methods:

  • Two-dimensional (2-D) gel electrophoresis with immobilized pH gradient (IPG) technology.
  • Protein identification using matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) and Mascot search.
  • Comparison of identified proteins against Escherichia coli and S. flexneri 2a genome databases.

Related Experiment Videos

Main Results:

  • Analysis of 488 protein spots, with 388 proteins identified, representing 169 genes.
  • Identification of one S. flexneri-specific protein.
  • Discovery of a potential gap in the S. flexneri 2a strain 2457T genome sequence.

Conclusions:

  • The generated proteome map serves as a valuable reference for future studies.
  • This research supports advancements in understanding S. flexneri pathogenicity.
  • The findings contribute to vaccine development and novel antibacterial drug design.