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Histopathologic changes in liver and renal tissues induced by Ochratoxin A and melatonin in rats
1Department of Pathology, Süleyman Demirel University, School of Medicine, Isparta, Turkey. gaydin@bigfoot.com
Abstract:
Nephrotoxicity and hepatotoxicity induced by Ochratoxin A (OTA) and ameliorating effects of melatonin were investigated in rats exposed to OTA. Experimental groups were as follows: control; OTA-treated; and OTA plus melatonin (MEL)-treated (OTA+MEL). The rats in the control group were administered with only a daily oral administration of 0.5 M NaHCO3. OTA was administered with a dose of 289 microg/kg in the same way. OTA and MEL were administered orally with OTA (289 microg/kg) and melatonin (10 mg/kg) in two different periods of time during the same day. The histopathologic changes in the liver and kidney tissues of control, OTA and OTA+MEL-treated rats were examined. There were no significant changes in the kidney and liver tissues of the control rats. Significant histopathologic changes were found in the kidney and liver tissue of rats treated with OTA. These were granular or vacuolated degeneration and necrosis of the liver cells, sinusoidal and central vein dilatation, bile duct proliferation, enlargement of periportal areas with mononuclear cell inflammatory infiltration and mild degree fibrous tissue proliferation, tubular epithelial cells degeneration, necrosis, proliferation and karyomegaly in the epithelial cells nuclei and peritubular and periglomerular lymphocyte infiltration, stromal fibrous tissue proliferation, hyperemic vessels. The severity of the lesions was significantly reduced by administration of melatonin. These results revealed that OTA induced significant histopathologic changes in liver and kidney tissue advocating OTA toxicity (P < 0.001), and administration of MEL+OTA significantly reduced the toxic effect of OTA on kidney and liver tissue of rats (P > 0.05).
Insights
Ochratoxin A (OTA) causes significant kidney and liver damage in rats. Melatonin administration effectively reduced these toxic effects, highlighting its protective potential against OTA toxicity.
Area of Science:
- Toxicology
- Pharmacology
- Pathology
Background:
- Ochratoxin A (OTA) is a mycotoxin known for its nephrotoxic and hepatotoxic effects.
- Investigating protective agents against OTA-induced organ damage is crucial for public health.
Purpose of the Study:
- To investigate the nephrotoxicity and hepatotoxicity induced by Ochratoxin A (OTA) in rats.
- To evaluate the ameliorating effects of melatonin (MEL) on OTA-induced organ damage.
Main Methods:
- Rats were divided into three groups: control, OTA-treated, and OTA + melatonin (MEL)-treated.
- Histopathological examination of liver and kidney tissues was performed to assess toxicity.
- Doses administered: OTA (289 microg/kg), Melatonin (10 mg/kg).
Main Results:
- OTA administration caused significant histopathological changes in the liver and kidneys, including cell degeneration, necrosis, inflammation, and fibrosis.
- Melatonin treatment significantly reduced the severity of OTA-induced lesions in both organs.
- OTA induced significant toxicity (P < 0.001), while MEL+OTA showed a significant reduction in toxic effects (P > 0.05).
Conclusions:
- Ochratoxin A is a potent nephrotoxin and hepatotoxin in rats.
- Melatonin demonstrates significant protective effects against Ochratoxin A-induced toxicity in the liver and kidneys.