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Updated: Sep 20, 2026

Setup of Capillary Electrophoresis-Inductively Coupled Plasma Mass Spectrometry (CE-ICP-MS) for Quantification of Iron Redox Species (Fe(II), Fe(III))
04:48

Setup of Capillary Electrophoresis-Inductively Coupled Plasma Mass Spectrometry (CE-ICP-MS) for Quantification of Iron Redox Species (Fe(II), Fe(III))

Published on: May 4, 2020

Does hepatomegaly alter iron-dependent oxidative effects in human plasma?

Monica Galleano1, Abraham Lemberg, Susana Puntarulo

  • 1Physical Chemistry-PRALIB, School of Pharmacy and Biochemistry, University of Buenos Aires, Argentina.

Human & Experimental Toxicology
|August 22, 2003
PubMed

A four-fold increase in the iron content of normal subjects was detected in plasma after 5 hours of iron administration. Iron supplementation had a surprisingly erratic effect on four patients with hepatomegaly secondary to heart insufficiency, since the increase in the iron content in the plasma after iron-dextran administration was either within the control range or significantly lower, independently of the initial values. Thiobarbituric acid reactive substances (TBARS) content was 2.5 +/- 0.2 and 4.3 +/- 0.4 microM for control and hepatomegalic subjects, respectively. The TBARS basal level was increased by iron supplementation. The difference between TBARS content in hepatomegalic and control subjects, after 5 hours of iron administration, was increased by 50% as compared to the difference in the basal content of TBARS. alpha-Tocopherol (alpha-T) content in plasma from subjects with hepatomegaly showed a significant decrease (-41%) as compared to control subjects. No significant difference over the basal level of alpha-T was measured after 5 hours of iron administration in any subject. The data presented here suggest that abnormal liver condition affects iron-dependent oxidative stress in plasma. Moreover, alpha-T does not seem to be the main antioxidant to control iron-dependent oxidative stress in plasma.

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