Related Experiment Videos
[Effects of fluoride on osteoclastic activity of rats in vitro]
Kun Hua1, Li-sha Bu, Guang-sheng Li
1Department of Pathology, Institute of Endemic Disease, Jilin University, Changchun 130021, China.
Objective:
To investigate the effects of fluoride on activities of tartrate-resistant acid phosphate (TRAP) and matrix metalloproteinase-9 (MMP-9) in rat osteoclasts cultured in vitro.
Methods:
Osteoclast was isolated mechanically from long bones of neonatal rats and cultured in vitro. Histochemical stain was applied to detect the effects of fluoride on activities of TRAP and in-situ hybridization was used to study the expression of MMP-9 mRNA in rat osteoclasts in vitro.
Results:
Number of TRAP positive cells was 154.2, 160.0, 170.6, 179.0 and 180.0 per cm(2), respectively for the rats with varied doses of fluoride, in a dose-response pattern but without statistical significance. The expression of MMP-9 mRNA increased with elevating dose of fluoride, especially in the rats with 1.00, 2.00 and 4.00 mg/L of fluoride, to 94.50, 94.64 and 104.97, respectively, significantly different from those in control group.
Conclusions:
Fluoride can enhance the MMP-9 mRNA expression in cultured osteoclasts of rats.