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Updated: Jul 12, 2026
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Murine Model of Wound Healing
Published on: May 28, 2013
A wound healing model using healing-impaired diabetic mice
R Tsuboi1, C M Shi, D B Rifkin
1Department of Dermatology, Juntendo University School of Medicine, Tokyo, Japan.
The Journal of Dermatology
|November 1, 1992
Summary
Basic fibroblast growth factor (bFGF) significantly improved wound healing in diabetic mice. Treatment with bFGF enhanced wound closure, granulation tissue, and vascularization in healing-impaired models.
Area of Science:
- Wound Healing Research
- Diabetic Complications
- Regenerative Medicine
Background:
- Diabetic mice exhibit impaired wound healing.
- Basic fibroblast growth factor (bFGF) plays a role in tissue repair.
- Understanding bFGF's efficacy in diabetic wound models is crucial.
Purpose of the Study:
- To quantitatively assess the effects of bFGF on wound healing in diabetic mice.
- To determine the optimal dosage and timing of bFGF application.
- To evaluate histological parameters of wound repair.
Main Methods:
- Utilized a quantitative histological approach in healing-impaired diabetic mice (C57BL KsJ db/db).
- Created full-thickness wounds and applied varying doses of bFGF.
- Assessed wound closure, granulation tissue thickness, matrix density, and capillary numbers at 8 days post-wounding.
Main Results:
- A 5-day application of 5 micrograms bFGF significantly improved all evaluated dermal parameters (p < 0.001).
- A minimum daily dose of 0.5 micrograms bFGF was required for significant effects.
- Granulation response peaked between 8-12 days, while matrix density increased up to 18 days.
Conclusions:
- bFGF demonstrates significant therapeutic potential for accelerating wound healing in diabetic conditions.
- Specific dosage and duration of bFGF treatment are critical for optimal outcomes.
- Histological analysis provides a robust method for evaluating bFGF's regenerative effects.
