Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Alpharetrovirus envelope-receptor interactions.

R J O Barnard1, J A T Young

  • 1McArdle Laboratories for Cancer Research, Department of Oncology, University of Wisconsin Madison, 1400 University Ave, Madison, WI 53706, USA.

Current Topics in Microbiology and Immunology
|August 23, 2003
PubMed
Summary

Avian sarcoma and leukosis virus (ASLV) uses a unique two-step entry mechanism. ASLV envelope glycoprotein (Env) requires receptor interaction before low pH triggers membrane fusion, unlike other viruses.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Anthrax toxin receptor 2 is expressed in murine and tumor vasculature and functions in endothelial proliferation and morphogenesis.

Oncogene·2009
Same author

Avian sarcoma and leukosis virus-receptor interactions: from classical genetics to novel insights into virus-cell membrane fusion.

Virology·2005
Same author

Low pH is required for avian sarcoma and leukosis virus Env-induced hemifusion and fusion pore formation but not for pore growth.

Journal of virology·2004
See all related articles

Area of Science:

  • Virology
  • Molecular Biology
  • Cellular Biology

Background:

  • Enveloped viruses infect cells through membrane fusion, mediated by viral fusion proteins.
  • Viral entry mechanisms are broadly classified as pH-dependent (e.g., influenza) or pH-independent (e.g., retroviruses).
  • Avian sarcoma and leukosis virus (ASLV) presents a novel entry mechanism combining features of both pH-dependent and independent pathways.

Purpose of the Study:

  • To review current research on ASLV envelope glycoprotein (Env)-receptor interactions.
  • To elucidate the molecular requirements for ASLV entry.
  • To describe the novel two-step entry mechanism of ASLV.

Main Methods:

  • Review of existing literature on ASLV entry mechanisms.
  • Analysis of ASLV Env-receptor interactions.

Related Experiment Videos

  • Focus on molecular requirements of viral fusion proteins and cognate receptors.

    • Main Results:

    • ASLV Env requires initial cell surface receptor binding to prime for fusion.
    • ASLV Env becomes sensitive to low pH only after receptor interaction.
    • This priming step is distinct from pH-dependent entry seen in other viruses.

    Conclusions:

    • ASLV employs a unique two-step viral entry process.
    • Receptor engagement is critical for priming ASLV Env for subsequent low-pH-induced fusion.
    • A model for ASLV Env-mediated membrane fusion is proposed.