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Related Experiment Videos

SarT influences sarS expression in Staphylococcus aureus.

Katherine A Schmidt1, Adhar C Manna, Ambrose L Cheung

  • 1Department of Microbiology, Dartmouth Medical School, Hanover, New Hampshire 03755, USA. Katherine.A.Schmidt@Dartmouth.edu

Infection and Immunity
|August 23, 2003
PubMed
Summary
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Staphylococcus aureus virulence is regulated by the sarA/agr network. This study reveals SarT acts as an intermediary, with SarT inducing sarS expression, which ultimately impacts protein A (spa) expression in this pathogen.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Bacterial Pathogenesis

Background:

  • Staphylococcus aureus virulence protein expression is controlled by regulatory loci like sarA and agr.
  • The sarA/agr network modulates adhesin and secreted toxin expression during bacterial growth phases.
  • SarA homologs, including sarT and sarS, are present in S. aureus, with sarA influencing their expression.

Purpose of the Study:

  • To elucidate the regulatory role of SarT and SarS in Staphylococcus aureus virulence.
  • To investigate the interaction between sarT, sarS, and the agr locus in controlling spa expression.
  • To determine the direct binding of SarT to the sarS promoter.

Main Methods:

  • Northern blot analysis of sarS and spa expression in wild-type and mutant S. aureus strains (sarT, sarT sarA, sarT agr).

Related Experiment Videos

  • Gel shift and footprinting assays to assess SarT binding to the sarS promoter.
  • Utilized a tetracycline-inducible system to control sarT expression in agr-activated strains.

    • Main Results:

    • SarA directly regulates spa expression.
    • The agr locus utilizes sarT as an intermediary to regulate sarS expression, leading to spa repression.
    • SarT directly binds to the sarS promoter, inducing sarS and spa expression in agr-positive strains.

    Conclusions:

    • SarT acts as a direct inducer of sarS expression.
    • The agr locus represses spa expression indirectly through the sarT-sarS regulatory pathway.
    • Understanding these regulatory interactions is crucial for controlling S. aureus virulence.