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Measuring Relative Insulin Secretion using a Co-Secreted Luciferase Surrogate
05:58

Measuring Relative Insulin Secretion using a Co-Secreted Luciferase Surrogate

Published on: June 25, 2019

The secreted glycoprotein CREG inhibits cell growth dependent on the mannose-6-phosphate/insulin-like growth factor

Alessandra Di Bacco1, Grace Gill

  • 1Department of Pathology, Harvard Medical School, 200 Longwood Ave., Boston, MA 02115, USA.

Oncogene
|August 23, 2003
PubMed

Insights

Cellular repressor of E1A-stimulated genes (CREG) inhibits cell growth by binding to the mannose-6-phosphate/insulin-like growth factor II receptor (M6P/IGF2R), a tumor suppressor. This interaction is crucial for CREG

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Cancer Research

Background:

  • Secreted proteins and receptors regulate cell functions like proliferation and differentiation.
  • Cellular repressor of E1A-stimulated genes (CREG) antagonizes cellular transformation and promotes neuronal differentiation.
  • Mannose-6-phosphate/insulin-like growth factor II receptor (M6P/IGF2R) is a tumor suppressor involved in cell growth control.

Purpose of the Study:

  • To investigate the mechanism of CREG-induced growth inhibition.
  • To determine the role of M6P/IGF2R in CREG's function.
  • To explore the interaction between CREG and M6P/IGF2R.

Main Methods:

  • Ectopic expression of CREG in NTERA-2 cells.
  • Cell cycle analysis to assess G1/S phase transition.
  • Assays to study CREG binding to M6P/IGF2R, including glycosylation dependence.
  • Analysis of CREG activity in M6P/IGF2R-deficient cells.

Main Results:

  • Ectopic CREG expression caused cell cycle delay at G1/S and growth inhibition.
  • CREG directly binds to M6P/IGF2R in a glycosylation-dependent manner.
  • M6P/IGF2R is essential for CREG-induced growth inhibition.

Conclusions:

  • CREG inhibits cell growth through a mechanism dependent on M6P/IGF2R.
  • The interaction between CREG and M6P/IGF2R suggests a role in regulating proliferation and differentiation.
  • CREG and M6P/IGF2R may collaborate in controlling cell growth across various lineages.

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