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[Hela cells secrete interleukin-8 and interleukin-10 response to Chlamydia trachomatis entry]
Jun-long Yu1, Ping Yu, Li-xin Li
1Department of Microbiology, Xiangya School of Medicine, Central South University, Changsha 410078, China.
Objective:
To investigate the effect of Chlamydia trachomatis serotype K infection on productions of IL-8 and IL-10 in supernatant of cultured Hela cells.
Methods:
The levels of IL-8 and IL-10 productions were detected by cell culture method and ELISA assay.
Results:
The amount of IL-8 and IL-10 in the cell culture supernatant rose with the increasing doses of Chlamydia trachomatis. The concentrations of IL-8 and IL-10 began to increase obviously at 24 h, and reached their highest level at 72 h, and then decreased. Heat-inactivated Chlamydia trachomatis induced lower levels of IL-8 and IL-10 production than live bacteria in vitro.
Conclusion:
The Chlamydia trachomatis can induce the secretion of IL-8 and IL-10 of host cells in a dose and time dependent manner and the intracellar replication of the bacteria is required for enhancing IL-8 and IL-10 productions by the infected cells.