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[Distribution and metabodynamics of hepatocyte targeting vector molecules in vivo]
Chun-hui Ouyang1, Fang-gen Lu, Dong-ye Yang
1Department of Gastroenterology, Second Xiangya Hospital, Central South University, Changsha 410011, China.
Objective:
To testify whether asiaorosomucoid (ASOR), the ligamental molecule of specific hepatocyte receptor prepared in our laboratory, can be used as a delivery drug vector for targeting hepatocyte.
Methods:
ASOR molecules marked with isotope 125I were injected into Strague-Dawley rats, and the distribution of ASOR on both organ and cellular levels was detected.
Results:
ASOR could only combine with hepatocyte and distribute in the liver. It did not combine with other important organ cells, such as the heart, brain, lung, spleen, kidney, bone and germen. The metabodynamics included: 10 mins after the injection, 90% ASOR was located in the liver, indicating that ASOR had a high hepatotropic; the metabodynamic equation was Ct = 66221e-3.362t + 8869e-0.2343t; the former half-life (T1/2 alpha was 0.206 h, and the latter half-life (T1/2 beta) was 5.575 h. The catabolite peptide of ASOR in the liver had affinity with the stomach.
Conclusion:
ASOR could have the qualification of being a drug targeting vector for the liver and is worth further research.