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Updated: Sep 20, 2026
Isolation of Atrial Myocytes from Adult Mice
Published on: July 25, 2019
[Encapsulation of atrial natriuretic peptide (ANP) cDNA transfection cells and ANP circadian expression]
Objective:
A technique based on the release of atrial natriuretic peptide (ANP) from encapsulated ANP cDNA transfected Chinese hamster ovary (CHO) cells for potential therapeutic approach to hypertension or congestive heart failure (CHF) was investigated.
Method:
ANP cDNA transfected CHO cells were encapsulated in polycaprolactone (PCL) tubes and the levels of ANP secreted from PCL tubes were detected. Circadian rhythm of ANP secreted by encapsulated transfected cells was also studied by regulation with melatonin.
Result:
During culturing, the encapsulated cells remained viable and the mean level of ANP reached 246.1 pg/ml/24 h in 2 ml medium containing one PCL tube (20 mm in length and 3 mm in diameter), whereas the secretion by the control was negative. The secretion of ANP showed a circadian variation: higher at night, but lower at day. The acrophase of circadian rhythm was 4:18 and shifted to 7:56 after melatonin was given.
Conclusion:
The above results demonstrated a potential use of the encapsulation technique of gene transfected cells implanted into human body for treatment of hypertension or CHF.
