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Related Experiment Videos

Cardiac dilatation associated with collagen alterations.

J B Caulfield1, P Norton, R D Weaver

  • 1University of Alabama, Department of Pathology, Birmingham 35294-0019.

Molecular and Cellular Biochemistry
|December 16, 1992
PubMed
Summary

Disrupting the heart's collagen network with disulfide reagents did not affect contractility but caused significant ventricular dilation. The collagen weave complex is crucial for maintaining the heart's structural integrity and pressure-volume relationship.

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Area of Science:

  • Cardiovascular Biology
  • Biochemistry
  • Structural Biology

Background:

  • The heart possesses a complex collagen network crucial for its mechanical function.
  • Previous studies have assigned functions to collagen components based on structure and location.
  • Demonstrating specific functional roles within this hierarchical network remains challenging.

Purpose of the Study:

  • To investigate the functional role of specific collagen components in the heart.
  • To determine the impact of disrupting the cardiac collagen network on ventricular mechanics.
  • To elucidate the contribution of the collagen weave complex to pressure-volume dynamics.

Main Methods:

  • Intravenous infusions of disulfide reagents to activate a collagenolytic system.

Related Experiment Videos

  • Assessment of cardiac collagen structure, myocyte contractility, and force generation.
  • Evaluation of ventricular pressure-volume relationships and distensibility.

    • Main Results:

    • Disulfide treatment led to near-complete loss of collagen struts interconnecting myocytes and capillaries, and the myocyte-surrounding weave complex.
    • No significant changes were observed in myocyte contractility or force delivery under increased preload or afterload.
    • Disulfide-treated hearts exhibited marked ventricular dilatation and increased distensibility, with a rightward shift in pressure-volume curves.

    Conclusions:

    • The cardiac collagen network, particularly the weave complex, plays a critical role in maintaining ventricular stiffness and shape.
    • Disruption of this network impairs passive diastolic properties without affecting active contractile function.
    • The weave complex significantly contributes to the initial rectilinear phase of the pressure-volume curve, indicating its importance in diastolic function.