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A chromatographic approach to analyze dansyl amino acid-HP-beta-CD association using macrocyclic antibiotic as the

C André1, M Thomassin, T T Truong

  • 1Laboratoire de Chimie Analytique, Faculté de Médecine et de Pharmacie, Place Saint-Jacques, 25030 Besançon cedex, France.

Journal of Chromatographic Science
|August 26, 2003
PubMed

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Affinity Chromatography

Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...